Crystalline forms of a fatty acid bile acid conjugate, preparation method thereof and use thereof

ABSTRACT

The present disclosure relates to novel crystalline forms of compound (I) and processes for preparation and use thereof. Crystalline forms CS3, CS2, CS5 and CS8 of compound (I) of the present disclosure have advantages in at least one aspect of solubility, stability, melting point, hygroscopicity, particle size, bioavailability, processability, purification effect, flowability, adhesiveness, stability in drug product, in vitro and in vivo dissolution, etc., which provides new and better choices for preparation of drug product containing compound (I) and has significant values for future drug development.

TECHNICAL FIELD

The present disclosure relates to the field of pharmaceutical polymorph,particularly relates to novel crystalline forms of a fatty acid bileacid conjugate, processes for preparation and use thereof. The presentdisclosure belongs to the field of medicine.

BACKGROUND

Aramchol is a fatty acid bile acid conjugate, specifically an amideconjugate of arachidic acid and 3-aminocholic acid. Aramchol caneffectively inhibit the activity of Stearoyl-CoA Desaturasel (SCD1)related to fatty acid metabolism modulation. By inhibiting SCD1,Aramchol reduces the synthesis of fatty acids and increases β-oxidation,leading to decrease of triglycerides and fatty acid esters in live,therefore, reducing liver fat content effectively as well as improvingmetabolic parameters associated with fatty liver disease. Aramcholdeveloped by Galmed Pharmaceuticals is used for treatment of fatty liverand Non Alcoholic Steatohepatitis in clinical development.

The chemical name of Aramchol is3β-arachidylamido-7α,12α-dihydroxy-5β-cholan-24-oic acid (hereinafterreferred to as “Compound (I)”), and the structure is shown as follows:

CN100386339C disclosed the process for preparing compound (I) withoutdisclosure of any solid form. The inventors of the present disclosurehave repeated the preparation method in CN100386339C and obtained anamorphous solid (the solid of the prior art). Further investigationindicates that the obtained amorphous solid has high hygroscopicity andpoor stability, with a weight gain of 2.44% under 80% RH (RelativeHumidity). Drugs substances with high hygroscopicity put a strictrequirements on packaging and storage and imposes higher costs, which isnot beneficial to production and applications. In order to overcomeabove-mentioned problems, the main objective of the present disclosureis to provide novel solid forms with excellent properties suitable fordrug development. Through enormous experiments and research, theinventors of the present disclosure discovered crystalline forms CS2,CS3, CS5 and CS8 of compound (I). These crystalline forms haveadvantages in at least one aspect of solubility, stability, meltingpoint, hygroscopicity, particle size, bioavailability, processability,purification effect, flowability, adhesiveness, formulation stability,in vitro and in vivo dissolution, etc. Particularly, the preparationmethods of the present disclosure are simple and the crystalline formshave low hygroscopicity, uniform particle size distribution andexcellent stability, which provides new and better choices forpreparation of drug containing compound (I) and is of great significancefor future drug development.

SUMMARY

According to the disadvantages of the prior art, the main objective ofthe present disclosure is to provide novel crystalline forms of compound(I), processes for preparation and use thereof.

According to the objective of the present disclosure, crystalline formCS3 of compound (I) is provided (hereinafter referred to as Form CS3),Form CS3 is an anhydrate.

The X-ray powder diffraction pattern of Form CS3 shows characteristicpeaks at 2theta values of 20.5°±0.2°, 15.4°±0.2° and 22.3°±0.2° usingCuKα radiation.

Furthermore, the X-ray powder diffraction pattern of Form CS3 shows oneor two or three characteristic peaks at 2theta values of 7.8°±0.2°,17.0°±0.2°, 17.9°±0.2°. Preferably, the X-ray powder diffraction patternof Form CS3 shows three characteristic peaks at 2theta values of7.8°±0.2°, 17.0°±0.2°, 17.9°±0.2°.

Furthermore, the X-ray powder diffraction pattern of Form CS3 shows oneor two or three characteristic peaks at 2theta values of 8.4°±0.2°,5.1°±0.2° and 19.1°±0.2°. Preferably, the X-ray powder diffractionpattern of Form CS3 shows three characteristic peaks at 2theta values of8.4°±0.2°, 5.1°±0.2° and 19.1°±0.2°.

Without any limitation being implied, the X-ray powder diffractionpattern of Form CS3 shows one or two or three or four or five or six orseven or eight or nine characteristic peaks at 2theta values of20.5°±0.2°, 15.4°±0.2°, 22.3°±0.2°, 7.8°±0.2°, 17.0°±0.2°, 17.9°±0.2°,8.4°±0.2°, 5.1°±0.2°, 19.1°±0.2° using CuKα radiation.

Without any limitation being implied, in a specific embodiment, theX-ray powder diffraction pattern of Form CS3 is substantially asdepicted in FIG. 1A.

According to the objective of the present disclosure, a process forpreparing Form CS3 is also provided, wherein the process comprises:

1) Suspending solid of compound (I) into a solvent mixture comprisingalcohols and water or alcohols and esters, then, stirring the solutionat 0-80° C. to obtain Form CS3. Preferably, said mixture of alcohols andwater is a mixture of isopropanol and water, said mixture of alcoholsand esters is a mixture of isopropanol and isopropyl acetate; or

2) Adding solid of compound (I) into a solvent to obtain a solution ofcompound (I), and adding an anti-solvent slowly into the solution ofcompound (I), wherein the solvent is halogenated hydrocarbons, and theanti-solvent is aromatic hydrocarbons; Or adding the solution ofcompound (I) into an anti-solvent, wherein the solvent is ethers, andthe anti-solvent is alkanes, stirring at room temperature to obtain FormCS3.

Preferably, said halogenated hydrocarbon is chloroform, said aromatichydrocarbon is toluene, said ether is tetrahydrofuran or2-methyltetrahydrofuran, said alkane is n-heptane.

Form CS3 of the present disclosure has at least one of the followingadvantages:

1) Compared with prior art, Form CS3 of the present disclosure is almostnon hygroscopic. The weight gain of the solid of prior art at 80% RH is2.44%, and the solid of prior art has high hygroscopicity. The weightgain of Form CS3 of the present disclosure at 80% RH is 0.15%. Due tothe low hygroscopicity, weight change and uncertainty of drug substancecontent caused by water absorption can be avoided, which is beneficialto long-term storage of drug and reduces the cost of material storageand quality control. Due to the low hygroscopicity, instability duringdrug preparation and/or storage and the un-processability of formulationcaused by external factors such as environmental moisture can beavoided. Low hygroscopicity is advantageous for accurate quantificationand later transportation and storage of the drug.

2) Compared with prior art, Form CS3 of the present disclosure hassuitable and uniform particle size distribution. The average particlesize of Form CS3 is smaller than 400 μm. Preferably, the averageparticle size of Form CS3 is smaller than 200 μm. Preferably, theaverage particle size of Form CS3 is smaller than 100 μm. In a specificembodiment, the average particle size of Form CS3 is 26.3 μm, D90 (thesize in microns below which 90 percent of the particles reside on avolume basis) is 62.8 μm. The average particle size of prior art is452.5 μm and D90 is 870.3 μm. Compared with prior art, Form CS3 has asmaller particle size, which increases the specific surface area of thedrug substance, improves the dissolution rate of drug, therebyfacilitating drug absorption and further improving the bioavailabilityof the drug. Moreover, compared with prior art, Form CS3 has a narrowerparticle size distribution and a monodisperse normal distribution,indicating that the powder of Form CS3 is highly homogeneous. API withuniform particle size distribution can be used in the formulationprocess directly, which avoids the complex pretreatment process,simplifies the production process, reduces cost, improves the uniformityof drug products, and makes the quality of pharmaceutical preparationsmore controllable.

3) Form CS3 of the present disclosure has good stability. Thecrystalline form of Form CS3 doesn't change for at least 3 weeks whenstored in open dishes under the condition of 25° C./60% RH, 40° C./75%RH and/or 60° C./75% RH. Preferably, doesn't change for at least 6months. Preferably, doesn't change for at least 1 year. Form CS3 hasexcellent stability, which can ensure that the quality of the drug willnot be affected as polymorphic transition will not occur in the processof preparation, transportation and storage. It is of great significanceto ensure the efficacy and safety of the drug and prevent the occurrenceof adverse drug reactions.

According to the objective of the present disclosure, crystalline formCS2 of Compound (I) is provided (hereinafter referred to as Form CS2).

The X-ray powder diffraction pattern of Form CS2 shows characteristicpeaks at 2theta values of 20.5°±0.2°, 16.8°±0.2°, 4.3°±0.2° using CuKαradiation.

Furthermore, the X-ray powder diffraction pattern of Form CS2 shows oneor two or three characteristic peaks at 2theta values of 8.4°±0.2°,17.6°±0.2°, 14.9°±0.2°. Preferably, the X-ray powder diffraction patternof Form CS2 shows three characteristic peaks at 2theta values of8.4°±0.2°, 17.6°±0.2°, 14.9°±0.2°.

Without any limitation being implied, the X-ray powder diffractionpattern of Form CS2 shows one or two or three or four or five or sixcharacteristic peaks at 2theta values of 20.5°±0.2°, 16.8°±0.2°,4.3°±0.2°, 8.4°±0.2°, 17.6°±0.2°, 14.9°±0.2° using CuKα radiation.Without any limitation being implied, in a specific embodiment, theX-ray powder diffraction pattern of Form CS2 is substantially asdepicted in FIG. 2A. Without any limitation being implied, Form CS2 hasisomorphism.

According to the objective of the present disclosure, a process forpreparing Form CS2 is also provided. Wherein the process comprises:

-   -   1) Mixing solid of compound (I) with methanol or a solvent        mixture of ethanol and acetone, filtering after dissolved,        storing the filtrate in a larger sealed device containing        acetonitrile at 0-30° C. to obtain Form CS2; or    -   2) Suspending solid of compound (I) into acetonitrile, then        stirring the solution at 0-30° C. to obtain Form CS2.

Form CS2 of the present disclosure has at least one of the followingadvantages:

1) Compared with prior art, Form CS2 of the present disclosure has lowerhygroscopicity. The weight gain of the solid of prior art under 80% RHis 2.44%, and the solid of prior art has high hygroscopicity. The weightgain of Form CS2 of the present disclosure at 80% RH is 0.46%. Due tothe low hygroscopicity, weight change and uncertainty of drug substancecontent caused by water absorption can be avoided, which is beneficialto long-term storage of drug and reduces the cost of material storageand quality control. Due to the low hygroscopicity, instability duringdrug preparation and/or storage and the un-processability of formulationcaused by external factors such as environmental moisture can beavoided. Low hygroscopicity is advantageous for accurate quantificationand later transportation and storage of the drug.

2) Compared with prior art, Form CS2 of the present disclosure hassuitable particle size and good uniformity. Average particle size ofForm CS2 is less than 400 μm. Preferably, the average particle size ofForm CS2 is less than 200 μm. Preferably, the average particle size ofForm CS2 is less than 100 μm. In a specific embodiment, the averageparticle size of Form CS2 is 41.9 μm, D90 (the size in microns belowwhich 90 percent of the particles reside on a volume basis) is 87.5 μm,but the average particle size of prior art is 452.5 μm, D90 is 870.3 μm.Compared with prior art, Form CS2 has a smaller crystal size, which canincrease the specific surface area of drugs, improve the dissolutionrate of drugs, facilitate drug absorption, and thus improvingbioavailability. Moreover, compared with prior art, Form CS2 has anarrower particle size distribution and a monodisperse normaldistribution, indicating that the powder of Form CS2 is highlyhomogeneous. API with uniform particle size distribution can be directlyapplied in the preparation process, which avoiding the complexpretreatment process of API, simplifying the process, reducing theproduction cost, improving the uniformity of pharmaceuticalpreparations, and making the quality of pharmaceutical preparations morecontrollable.

3) Form CS2 of the present disclosure has good stability. The Form CS2doesn't change for at least 3 weeks when stored in open dishes under thecondition of 25° C./60% RH or 40° C./75% RH or 60° C./75% RH.Preferably, doesn't change for at least 3 months. Preferably, doesn'tchange for at least 6 months. Preferably, doesn't change for at least 1year. Form CS2 has excellent stability, which can ensure that thequality of the drug will not be affected as polymorphic transition willnot occur in the process of preparation, transportation and storage. Itis of great significance to ensure the efficacy and safety of the drugand prevent the occurrence of adverse drug reactions.

According to the objective of the present disclosure, crystalline formCS5 of Compound (I) is provided (hereinafter referred to as Form CS5).

The X-ray powder diffraction pattern of Form CS5 shows characteristicpeaks at 2theta values of 4.8°±0.2°, 9.7°±0.2°, 6.4°±0.2° using CuKαradiation.

Furthermore, the X-ray powder diffraction pattern of Form CS5 shows oneor two or three or four characteristic peaks at 2theta values of20.9°±0.2°, 16.8°±0.2°, 23.3°±0.2°, 14.6°±0.2°. Preferably, the X-raypowder diffraction pattern of Form CS5 shows three characteristic peaksat 2theta values of 20.9°±0.2°, 16.8°±0.2°, 23.3°±0.2°, 14.6°±0.2°.

Without any limitation being implied, the X-ray powder diffractionpattern of Form CS5 shows one or two or three or four or five or six orseven characteristic peaks at 2theta values of 4.8°±0.2°, 9.7°±0.2°,6.4°±0.2°, 20.9°±0.2°, 16.8°±0.2°, 23.3°±0.2°, 14.6°±0.2° using CuKαradiation.

Without any limitation being implied, in a specific embodiment of thepresent disclosure, Form CS5 is a hydrate, and the X-ray powderdiffraction pattern of Form CS5 is substantially as depicted in FIG. 3A.

According to the objective of the present disclosure, a process forpreparing Form CS5 is also provided. The process comprises: Adding solidof compound (I) into a solvent selected from alcohols to obtain asolution of compound (I), and adding acetonitrile slowly into thesolution of compound (I), stirring at room temperature until solidprecipitates, then, separating and drying to obtain Form CS5.Preferably, said alcohols are methanol, ethanol and isopropanol.

Form CS5 of the present disclosure has at least one of the followingadvantages:

1) Compared with prior art, Form CS5 of the present disclosure has lowerhygroscopicity. Weight gain of solid of prior art under 80% RH is 2.44%,and the hygroscopicity is high. The weight gain of Form CS5 of thepresent disclosure at 80% RH is 0.35%. Due to the low hygroscopicity,weight change and uncertainty of drug substance content caused by waterabsorption can be avoided, which is beneficial to long-term storage ofdrug and reduces the cost of material storage and quality control. Dueto the low hygroscopicity, instability during drug preparation and/orstorage and the un-processability of formulation caused by externalfactors such as environmental moisture can be avoided. Lowhygroscopicity is advantageous for accurate quantification and latertransportation and storage of the drug.

2) Compared with prior art, Form CS5 of the present disclosure hassuitable particle size and good uniformity. Average particle size ofForm CS5 is less than 400 μm. Preferably, the average particle size ofForm CS5 is less than 200 μm. Preferably, the average particle size ofForm CS5 is less than 100 μm. In a specific embodiment, the averageparticle size of Form CS5 is 75.6 μm, but the average particle size ofprior art is 452.5 μm. Compared with prior art, Form CS5 has a smallercrystal size, which can increase the specific surface area of drugs,improve the dissolution rate of drugs, facilitate drug absorption, andthus improving bioavailability. Moreover, compared with prior art, FormCS5 has a narrower particle size distribution and a monodisperse normaldistribution, indicating that the powder of Form CS5 is highlyhomogeneous. API with uniform particle size distribution can be directlyapplied in the preparation process, which avoiding the complexpretreatment process of API, simplifying the process, reducing theproduction cost, improving the uniformity of pharmaceuticalpreparations, and making the quality of pharmaceutical preparations morecontrollable.

3) Form CS5 of the present disclosure has good stability. The Form CS5doesn't change for at least 3 weeks when stored in open dishes under thecondition of 25° C./60% RH or 40° C./75% RH or 60° C./75% RH.Preferably, doesn't change for at least 3 months. Preferably, doesn'tchange for at least 6 months. Preferably, doesn't change for at least 1year. Form CS5 has excellent stability, which can ensure that thequality of the drug will not be affected as polymorphic transition willnot occur in the process of preparation, transportation and storage. Itis of great significance to ensure the efficacy and safety of the drugand prevent the occurrence of adverse drug reactions.

According to the objective of the present disclosure, crystalline formCS8 of Compound (I) is provided (hereinafter referred to as Form CS8).

The X-ray powder diffraction pattern of Form CS8 shows characteristicpeaks at 2theta values of 6.0°±0.2°, 15.5°±0.2°, 20.7°±0.2° using CuKαradiation.

Furthermore, the X-ray powder diffraction pattern of Form CS8 shows oneor two or three or four characteristic peaks at 2theta values of21.8°±0.2°, 9.1°±0.2°, 19.9°±0.2°, 22.9°±0.2°. Preferably, the X-raypowder diffraction pattern of Form CS8 shows four characteristic peaksat 2theta values of 21.8°±0.2°, 9.1°±0.2°, 19.9°±0.2°, 22.9°±0.2°.

Without any limitation being implied, the X-ray powder diffractionpattern of Form CS8 shows one or two or three or four or five or six orseven characteristic peaks at 2theta values of 6.0°±0.2°, 15.5°±0.2°,20.7°±0.2°, 21.8°±0.2°, 9.1°±0.2°, 19.9°±0.2°, 22.9°±0.2° using CuKαradiation.

Without any limitation being implied, in a specific embodiment of thepresent disclosure, the X-ray powder diffraction pattern of Form CS8 issubstantially as depicted in FIG. 4A.

According to the objective of the present disclosure, a process forpreparing Form CS8 is also provided. The process comprises:

Storing Form CS2 at room temperature for 7-30 days, heating the obtainedsample to 95° C.±2° C. at a rate of 5-20° C./min with nitrogen purging,holding at 95° C.±2° C. for 1-10 min, and then cooling to roomtemperature at a rate of 5-20° C./min to obtain Form CS8.

Form CS8 of the present disclosure has at least one of the followingadvantages:

1) Compared with prior art, Form CS8 of the present disclosure has lowera lower hygroscopicity. Weight gain of solid of prior art under 80% RHis 2.44%, and the hygroscopicity is high. The weight gain of Form CS8 ofthe present disclosure at 80% RH is 0.40%. Due to the lowhygroscopicity, weight change and uncertainty of drug substance contentcaused by water absorption can be avoided, which is beneficial tolong-term storage of drug and reduces the cost of material storage andquality control. Due to the low hygroscopicity, instability during drugpreparation and/or storage and the un-processability of formulationcaused by external factors such as environmental moisture can beavoided. Low hygroscopicity is advantageous for accurate quantificationand later transportation and storage of the drug.

2) Compared with prior art, Form CS8 of the present disclosure hassuitable particle size, and has good uniformity. Average particle sizeof Form CS8 is less than 400 μm. Preferably, the average particle sizeof Form CS8 is less than 200 μm. Preferably, the average particle sizeof Form CS8 is less than 100 μm. Form CS8 has a smaller crystal size,which can increase the specific surface area of drugs, improve thedissolution rate of drugs, facilitate drug absorption, and thusimproving bioavailability. Moreover, compared with prior art, Form CS8has a narrower particle size distribution and a monodisperse normaldistribution, indicating that the powder of Form CS8 is highlyhomogeneous. API with uniform particle size distribution can be directlyapplied in the preparation process, which avoiding the complexpretreatment process of API, simplifying the process, reducing theproduction cost, improving the uniformity of pharmaceuticalpreparations, and making the quality of pharmaceutical preparations morecontrollable.

3) Form CS8 of the present disclosure has good stability. The Form CS8doesn't change for at least 3 weeks when stored in open dishes under thecondition of 25° C./60% RH or 40° C./75% RH or 60° C./75% RH.Preferably, doesn't change for at least 3 months. Preferably, doesn'tchange for at least 6 months. Preferably, doesn't change for at least 1year. Form CS8 has good stability, and it is of great significance toensure the efficacy and safety of drugs and prevent the occurrence ofadverse drug reactions.

In processes for preparing crystalline forms of the present disclosure:

Said “room temperature” is 25° C.±5° C.

Said “stirring” is accomplished by using a conventional method in thefield such as magnetic stirring or mechanical stirring and the stirringspeed is 50 to 1800 r/min, preferably the magnetic stirring speed is 300to 900 r/min and mechanical stirring speed is 100 to 300 r/min.

Said “separation” is accomplished by using a conventional method in thefield such as centrifugation or filtration. The operation of“centrifugation” is as follows: the sample to be separated is placedinto the centrifuge tube, and then centrifuged at a rate of 10000 r/minuntil the solid all sink to the bottom of the tube.

Said “drying” is accomplished at room temperature or a highertemperature. The drying temperature is from room temperature to about60° C., or to 50° C., or to 40° C. The drying time can be 2 to 48 hours,or overnight. Drying is accomplished in a fume hood, oven or vacuumoven.

Said “evaporating” is accomplished by using a conventional method in thefield such as slow evaporation or rapid evaporation. For example,evaporation is accomplished in a container covered by sealing film withpinholes.

Said “cooling” is accomplished by using conventional methods in thefield such as slow cooling and rapid cooling. Slow cooling is usuallyaccomplished at the speed of 0.1° C./min. Isomorphous crystals areformed during the crystallization process, when molecules in somepositions are partially or completely replaced by other molecules withsimilar properties. The obtained isomorphous crystals are single phasecrystals. Isomorphism doesn't change the crystal structure orconnectivity of the crystal. There may be slight changes in unit cellparameters. As a result, the XRPD patterns of isomorphous crystals areidentical or similar.

In the present disclosure, “crystal” or “crystalline form” refers to thecrystal or the crystalline form being identified by the X-raydiffraction pattern shown herein. Those skilled in the art are able tounderstand that physicochemical properties discussed herein can becharacterized. The experimental errors depend on the instrumentconditions, the sampling processes and the purity of samples. Inparticular, those skilled in the art generally know that the X-raydiffraction pattern typically varies with the experimental conditions.It is necessary to point out that, the relative intensity of thediffraction peaks in the X-ray diffraction pattern may also vary withthe experimental conditions; therefore, the order of the diffractionpeak intensities cannot be regarded as the sole or decisive factor. Infact, the relative intensity of the diffraction peaks in the X-raypowder diffraction pattern is related to the preferred orientation ofthe crystals, and the diffraction peak intensities shown herein areillustrative and identical diffraction peak intensities are notrequired. In addition, the experimental error of the diffraction peakposition is usually 5% or less, and the error of these positions shouldalso be taken into account. An error of ±0.2° is usually allowed. Inaddition, due to experimental factors such as sample thickness, theoverall offset of the diffraction peak is caused, and a certain offsetis usually allowed. Thus, it will be understood by those skilled in theart that a crystalline form of the present disclosure is not necessarilyto have exactly the same X-ray diffraction pattern of the example shownherein. As used herein, “the same XRPD pattern” does not mean absolutelythe same, the same peak positions may differ by ±0.2° and the peakintensity allows for some variability. Any crystalline forms whose X-raydiffraction patterns have the same or similar characteristic peaksshould be within the scope of the present disclosure. Those skilled inthe art can compare the patterns shown in the present disclosure withthat of an unknown crystalline form in order to identify whether thesetwo groups of patterns reflect the same or different crystalline forms.

In some embodiments, crystalline Form CS2, CS3, CS5, CS8 of the presentdisclosure is pure and substantially free of any other crystallineforms. In the present disclosure, the term “substantially free” whenused to describe a novel crystalline form, it means that the content ofother crystalline forms in the novel crystalline form is less than 20%(w/w), specifically less than 10% (w/w), more specifically less than 5%(w/w) and further more specifically less than 1% (w/w).

It should be noted that the number and the number range should not beunderstood as the number or number range themselves only. It should beunderstood by those skilled in the art that the specific number can beshifted at specific technical environment without departing from thespirit and principle of the present disclosure. In the presentdisclosure, the number of shift ranges expected by one of skilled in theart is represented by the term “about”.

In addition, the present disclosure provides a pharmaceuticalcomposition, said pharmaceutical composition comprises a therapeuticallyand/or prophylactically effective amount of Form CS2, CS3, CS5 or CS8and pharmaceutically acceptable carriers, diluents or excipients.

Furthermore, Form CS2, CS3, CS5 or CS8 can be used for preparing drugstreating non-alcoholic steatohepatitis, gallstones, cholesterolgallstone and atherosclerosis.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1A shows an XRPD pattern of Form CS3 of the present disclosure.

FIG. 1B shows a TGA curve of Form CS3 of the present disclosure.

FIG. 1C shows a DSC curve of Form CS3 of the present disclosure.

FIG. 1D shows a ¹H NMR spectrum of Form CS3 of the present disclosure.

FIG. 1E shows an XRPD pattern overlay of Form CS3 of the presentdisclosure before and after being stored under 25° C./60% RH for oneyear, top: XRPD pattern before storage, bottom: XRPD pattern afterstorage.

FIG. 1F shows an XRPD pattern overlay of Form CS3 of the presentdisclosure before and after being stored under 40° C./75% RH for oneyear, top: XRPD pattern before storage, bottom: XRPD pattern afterstorage.

FIG. 1G shows a DVS plot of Form CS3 of the present disclosure.

FIG. 1H shows an XRPD pattern overlay of Form CS3 of the presentdisclosure before and after DVS test, top: XRPD pattern before test,bottom: XRPD pattern after test.

FIG. 1I shows a DVS plot of the prior art.

FIG. 1J shows a PSD diagram of Form CS3 of the present disclosure.

FIG. 1K shows a PSD diagram of the prior art.

FIG. 2A shows an XRPD pattern of Form CS2 of the present disclosure.

FIG. 2B shows a TGA curve of Form CS2 of the present disclosure.

FIG. 2C shows a DSC curve of Form CS2 of the present disclosure.

FIG. 2D shows an XRPD pattern overlay of Form CS2 of the presentdisclosure before and after DVS test, top: XRPD pattern before test,bottom: XRPD pattern after test.

FIG. 2E shows a PSD diagram of Form CS2 of the present disclosure.

FIG. 3A shows an XRPD pattern of Form CS5 of the present disclosure.

FIG. 3B shows a TGA curve of Form CS5 of the present disclosure.

FIG. 3C shows a DSC curve of Form CS5 of the present disclosure.

FIG. 3D shows a DVS plot of Form CS5 of the present disclosure.

FIG. 4A shows an XRPD pattern of Form CS8 of the present disclosure.

FIG. 4B shows a TGA curve of Form CS8 of the present disclosure.

FIG. 4C shows a DSC curve of Form CS8 of the present disclosure.

FIG. 4D shows an XRPD pattern overlay of Form CS8 of the presentdisclosure before and after being stored under 60° C./75% RH for 3weeks, top: XRPD pattern before storage, bottom: XRPD pattern afterstorage.

FIG. 4E shows a DVS plot of Form CS8 of the present disclosure.

FIG. 4F shows an XRPD pattern overlay of Form CS8 of the presentdisclosure before and after DVS test, top: XRPD pattern before test,bottom: XRPD pattern after test.

DETAILED DESCRIPTION

The present disclosure is further illustrated by the following exampleswhich describe the preparation and use of the crystalline forms of thepresent disclosure in detail. It is obvious to those skilled in the artthat many changes in the materials and methods can be accomplishedwithout departing from the scope of the present disclosure.

The abbreviations used in the present disclosure are explained asfollows:

-   -   XRPD: X-ray Powder Diffraction    -   DSC: Differential Scanning calorimetry    -   TGA: Thermal Gravimetric Analysis    -   DVS: Dynamic Vapor Sorption    -   ¹H NMR: Proton Nuclear Magnetic Resonance    -   PSD: Particle Size Distribution

X-ray powder diffraction patterns in the present disclosure wereacquired by a Bruker D2 PHASER X-ray powder diffractometer. Theparameters of the X-ray powder diffraction method of the presentdisclosure are as follows:

-   -   X-ray Reflection: Cu, Kα    -   Kα1 (Å): 1.54060; Kα2 (Å): 1.54439    -   Kα2/Kα1 intensity ratio: 0.50    -   Voltage: 30 (kV)    -   Current: 10 (mA)    -   Scan range: from 3.0 degree to 40.0 degree

Differential scanning calorimetry (DSC) data in the present disclosurewere acquired by a TA Q2000. The parameters of the DSC method of thepresent disclosure are as follows:

-   -   Heating rate: 10° C./min    -   Purge gas: nitrogen

Thermal gravimetric analysis (TGA) data in the present disclosure wereacquired by a TA Q500. The parameters of the TGA method of the presentdisclosure are as follows:

-   -   Heating rate: 10° C./min    -   Purge gas: nitrogen

Proton nuclear magnetic resonance spectrum data (¹H NMR) were collectedfrom a Bruker Avance II DMX 400M HZ NMR spectrometer. 1-5 mg of samplewas weighed and dissolved in 0.5 mL of deuterated dimethyl sulfoxide toobtain a solution with a concentration of 2-10 mg/mL.

The particle size distribution data in the present disclosure wereacquired by an S3500 laser particle size analyzer of Microtrac.Microtrac S3500 is equipped with an SDC (Sample Delivery Controller).The test was carried out in wet mode, and the dispersion medium isIsopar G. The parameters are as follows:

Size distribution: Volume Run Time: 10 s Dispersion medium: Isopar GParticle coordinates: Standard Run Number: 3 Fluid refractive index:1.42 Particle Transparency: Trans Residuals: Enabled Particle refractiveindex: 1.5 Flow rate: 60%* Particle shape: Irregular Filtration: EnabledUltrasonication power: 30 W Ultrasonication time: 30 s *Flow rate 60% is60% of 65 mL/s.

Unless otherwise specified, the following examples were conducted atroom temperature. Solid of compound (I) used in the following exampleswere prepared by known methods in the prior art, the method disclosed inCN100386339C.

Example 1

Preparation of Form CS3 Example 1

As shown in Table 1A, certain amount of compound (I) was weighed intoeach of three glass vials followed by adding corresponding volume ofsolvent to form a suspension. These suspensions were stirred at RT, thencentrifuged and dried. The obtained solid was collected and labeled assample 1-a, 1-b, 1-c.

TABLE 1A Sample ID Mass (mg) Solvent (v/v) Volume (mL) 1-a 10.6 IPA/H₂O1:1 0.4 1-b 10.4 EtOH/EtOAc 1:9 0.5 1-c 9.1 IPA/IPAc 1:1 0.4

Sample 1-a, 1-b, 1-c were confirmed to be Form CS3 by XRPD. The XRPDpattern of sample 1-a is depicted in FIG. 1A, and the XRPD data arelisted in Table 1B. The XRPD patterns of sample 1-b and 1-c are the sameas or similar to that of sample 1-a.

TABLE 1B 2θ d spacing Intensity % 5.10 17.31 21.16 5.44 16.24 16.26 6.5613.47 17.60 7.76 11.40 47.21 8.40 10.52 23.60 10.76 8.22 11.09 11.507.70 16.24 11.98 7.39 16.03 12.39 7.14 15.35 12.98 6.82 16.15 13.69 6.4728.31 14.08 6.29 41.22 15.07 5.88 19.39 15.41 5.75 54.37 15.75 5.6318.98 16.72 5.30 11.17 17.04 5.20 39.33 17.21 5.15 48.52 17.56 5.0515.91 17.94 4.94 28.29 18.42 4.82 11.18 18.76 4.73 20.05 19.17 4.6332.94 20.49 4.33 100.00 21.44 4.14 15.85 22.32 3.98 60.51 22.67 3.9234.67 23.28 3.82 11.51 23.73 3.75 11.35 24.33 3.66 10.58 25.14 3.5410.29 25.70 3.47 5.16 26.49 3.36 5.65

The TGA curve of Form CS3 shows about 0.3% weight loss when heated to120° C., which is depicted in FIG. 1B.

The DSC curve of Form CS3 is depicted in FIG. 1C, which shows oneendothermic peak at around 111° C. (onset temperature).

The ¹H NMR spectrum of Form CS3 is depicted in FIG. 1D, and thecorresponding data are: ¹HNMR (400 MHz, DMSO) δ 11.95 (s, 1H), 7.53 (d,J=7.0 Hz, 1H), 4.12 (d, J=3.3 Hz, 1H), 4.05 (d, J=3.2 Hz, 1H), 3.83 (s,1H), 3.77 (s, 1H), 3.61 (s, 1H), 2.26-1.93 (m, 6H), 1.89-1.03 (m, 53H),1.04-0.79 (m, 10H), 0.59 (s, 3H). Except water, no other solvent residuewas observed.

Preparation of Form CS3 Example 2:

15.6 mg of compound (I) was dissolved into 0.5 mL of CHCl₃. The solutionwas filtered and 2.0 mL of toluene was added into the filtrate. Then theclear solution was stirred until solid crystallized. The solid wascollected by centrifugation, and the obtained solid was dried andlabeled as sample 1-d. Sample 1-d was confirmed to be Form CS3.

Preparation of Form CS3 Example 3:

As shown in Table 1C, certain amount of compound (I) was dissolved incorresponding volume of solvent. After filtering, the filtrate was addedinto certain volume of anti-solvent. The suspension was stirred at RTuntil a large amount of solid precipitated. The solid was collected bycentrifugation, and the obtained solid was dried and labeled as sample1-e and 1-f. Sample 1-e and 1-f were confirmed to be Form CS3.

TABLE 1C Solvent Volume of Volume of Sample Mass Volume filtrate Anti-anti- solvent ID Method (mg) Solvent (mL) (mL) solvent (mL) 1-e Reverse15.6 THF 0.5 0.2 n-Heptane 2.0 anti-solvent addition 1-f Reverse 15.42-MeTHF 1.0 0.4 n-Heptane 2.0 anti-solvent addition

Stability Study of Form CS3

As active pharmaceutical ingredient plays an important part in drugproducts, it is vital that the crystalline active pharmaceuticalingredient has good physical and chemical stability. Good physicalstability avoids crystal transformation during the storage andformulation processes, thereby ensuring consistent and controllablequality of the drug substance and drug product.

Solid samples of Form CS3 were stored under different conditions of 25°C./60% RH and 40° C./75% RH in open dishes for one year. Crystallineform was checked by XRPD. The results are shown in Table 1D.

TABLE 1D Initial crystalline form Conditions Time Form change Form CS325° C./60% RH One No form change (FIG. 1E top) year observed (FIG. 1Ebottom) Form CS3 40° C./75% RH One No form change (FIG. 1F top) yearobserved (FIG. 1F bottom)

The results show that Form CS3 has good stability and meets therequirements of the guidance of stability testing of drug substances andproduces of China and/or American. Therefore, Form CS3 is suitable fordrug development.

Hygroscopicity Study of Form CS3

Dynamic vapor sorption (DVS) was applied to test hygroscopicity of FormCS3 and the solid disclosed in CN100386339C with about 15 mg of samples.The results are listed in Table 1E. As shown in FIG. 1I, the soliddisclosed in prior art is hygroscopic with 2.44% weight gain under 80%RH. While Form CS3 is non hygroscopic or almost non hygroscopic with0.15% weight gain under 80% RH. DVS plot of test hygroscopicity of FormCS3 is depicted in FIG. 1G. What's more, the crystalline form of FormCS3 didn't change after DVS test, which is depicted in FIG. 1H.

TABLE 1E Weight gain under 80% The definition of Sample RelativeHumidity hygroscopicity Form CS3 0.15% non hygroscopic or almost nonhygroscopic Solid disclosed in 2.44% hygroscopic CN100386339C

Description and definition of hygroscopicity (Chinese Pharmacopoeia 2015edition appendix XIX J Drug hygroscopic test guidelines, test at 25°C.+/−1° C., 80% RH.).

-   -   deliquescent: Sufficient water is absorbed to form a liquid;    -   very hygroscopic: Increase in mass is equal to or greater than        15 percent;    -   hygroscopic: Increase in mass is less than 15 percent and equal        to or greater than 2 percent;    -   slightly hygroscopic: Increase in mass is less than 2 percent        and equal to or greater than 0.2 percent.    -   non hygroscopic or almost non hygroscopic: Increase in mass is        less than 0.2 percent.

Particle Size Study of Form CS3

The particle size distribution of Form CS3 and the solid disclosed inCN100386339C were tested after ultrasonication for 30 seconds. Theresults are shown in Table 1F. The average particle size of Form CS3 is26.3 μm, and D90 is 62.8 μm. The average particle size and D90 of thesolid disclosed in prior art are 452.5 μm and 870.3 μm. Compared withprior art, Form CS3 has smaller particle size, which increases thespecific surface area of the drug substance, improves the dissolutionrate of drug, thereby facilitating drug absorption and further improvingthe bioavailability of the drug.

TABLE 1F Sample MV (μm) D10 (μm) D50 (μm) D90 (μm) Form CS3 26.3 2.012.3 62.8 Solid disclosed in 452.5 95.0 398.8 870.3 CN100386339C

Explanation of the abbreviations used in the present invention is asfollows:

MV: Average particle diameter calculated by volume.

D10: the size in microns below which 10 percent of the particles resideon a volume basis.

D50: the size in microns below which 50 percent of the particles resideon a volume basis, also known as the median diameter.

D90: the size in microns below which 90 percent of the particles resideon a volume basis.

The particle size distribution diagrams of Form CS3 and the soliddisclosed in prior art are shown in FIGS. 1J and 1K. As shown in FIG.1J, Form CS3 has a narrow, nearly normal and uniform. Whereas, the soliddisclosed in prior art has a large particle size and poor uniformity.

Example 2

Preparation of Form CS2 Example 1:

As shown in Table 2A, certain amount of compound (I) was dissolved incorresponding volume of solvent, and then filtered. The glass vial withfiltrate was placed into a 20 mL glass vial with 5.0 mL of acetonitrile(ACN). The 20 mL glass vial was sealed and placed until solidcrystallized. The obtained solids were labeled as sample 2-a˜2-c.

TABLE 2A Sample ID Mass (mg) Solvent (v/v) Volume (mL) 2-a 32.6EtOH/Acetone 1:1 1.5 2-b 55.9 EtOH/Acetone 1:1 2.0 2-c 25.7 MeOH 1.4

Preparation of Form CS2 Example 2:

Approximately 5.3 mg of compound (I) was weighed into a 3 mL glass vialfollowed by adding 2.0 mL of ACN to form a suspension. The suspensionwas stirred at RT for 5 days and centrifuged. The obtained solids werelabeled as sample 2-d.

Sample 2-a˜2-d were confirmed to be Form CS2 by XRPD. The XRPD patternof sample 2-a is depicted in FIG. 2A, and the XRPD data are listed inTable 2B. The XRPD patterns of sample 2-b˜2-d are the same as or similarto that of sample 2-a.

TABLE 2B 2θ d spacing Intensity % 2.91 30.32 8.71 4.27 20.70 32.29 4.5419.48 37.12 5.95 14.86 3.73 8.45 10.47 27.43 8.84 10.00 5.88 10.35 8.544.46 12.25 7.22 3.82 14.32 6.18 19.12 14.52 6.10 22.19 14.94 5.93 12.7816.81 5.27 56.15 17.45 5.08 17.20 17.90 4.96 8.29 20.65 4.30 100.0020.85 4.26 71.33 23.50 3.79 6.83 25.84 3.45 5.63 28.24 3.16 1.35 32.262.78 1.11 34.20 2.62 3.69 34.82 2.58 2.26

The TGA curve of Form CS2 shows about 2.9% weight loss when heated to100° C., which is depicted in FIG. 2B. Form CS2 is a hydrate.

The DSC curve of Form CS2 is depicted in FIG. 2C, which shows oneendothermic peak at around 83° C. (onset temperature).

Hygroscopicity Study of Form CS2

Dynamic vapor sorption (DVS) was applied to test hygroscopicity of FormCS2 with about 10 mg of samples. The results are listed in Table 2C andthe XRPD patterns before and after DVS are shown in FIG. 2D. The resultshows that weight gain of Form CS2 under 80% RH is 0.46%. Form CS2 isslightly hygroscopic according to the Chinese Pharmacopoeia 2015 editionappendix XIX J Drug hygroscopic test guidelines. As depicted in FIG. 1I,weight gain of the solid disclosed in prior art under 80% RH is 2.44%.The hygroscopicity of Form CS2 is lower than that of prior art. No formchange of Form CS2 was observed before and after DVS test.

TABLE 2C Weight gain under 80% The definition of Sample RelativeHumidity hygroscopicity Form CS2 0.46% slightly hygroscopic Soliddisclosed in 2.44% hygroscopic CN100386339C

Particle Size Study of Form CS2

The particle size distribution of Form CS2 and the solid disclosed inCN100386339C were tested after ultrasonication for 30 seconds. Theresults are shown in Table 2D. An average particle size of Form CS2 is41.9 μm, and D90 is 87.5 μm, while the average particle size and D90 ofthe solid disclosed in prior art are 452.5 μm and 870.3 μm. Comparedwith prior art, Form CS2 has smaller particle size, which increases thespecific surface area of the drug substance, improves the dissolutionrate of drug, thereby facilitating drug absorption and further improvingthe bioavailability of the drug.

TABLE 2D Sample MV (μm) D10 (μm) D50 (μm) D90 (μm) Form CS2 41.9 9.727.9 87.5 Solid disclosed in 452.5 95.0 398.8 870.3 CN100386339C

The particle size distribution diagrams of Form CS2 and the soliddisclosed in prior art are shown in FIGS. 2E and 1K. As shown in FIG.2E, Form CS2 has a narrow, nearly normal and uniform. Whereas, the soliddisclosed in prior art has a large particle size and poor uniformity.

Example 3

Preparation of Form CS5 Example 1:

As shown in Table 3A, certain amount of compound (I) was dissolved incorresponding volume of solvent and then filtered. Then anti-solvent wasadded into the filtrate drop by drop until a large amount of solidprecipitated. The suspension was then centrifuged and solid wasobtained. The solid were dried at RT and labeled as sample 3-a˜3-c.Sample 3-a˜3-c were confirmed to be Form CS5 by XRPD. The XRPD patternof sample 3-a is depicted in FIG. 3A, and the XRPD data are listed inTable 3B. The XRPD pattern of sample 3-b and 2-c are the same as orsimilar to that of sample 3-a.

TABLE 3A Volume of Volume of Volume of Sample Mass Solvent solventfiltrate Anti- anti-solvent ID (mg) ( v/v ) (mL) (mL) solvent (mL) 3-a100.5 EtOH 2.0 2.0 ACN 10.0 3-b 92.2 MeOH 4.8 0.8 ACN 3.0 3-c 15.8 IPA0.5 0.2 ACN 2.0

TABLE 3B 2θ d spacing Intensity % 3.11 28.41 35.83 3.34 26.43 36.32 4.7618.57 100.00 5.12 17.26 56.39 6.43 13.74 25.69 6.88 12.85 14.22 7.9011.19 9.13 9.71 9.11 26.62 10.43 8.48 15.50 11.31 7.82 6.40 12.11 7.314.84 13.96 6.34 7.31 14.56 6.08 11.44 16.34 5.42 10.45 16.82 5.27 15.5017.86 4.97 10.06 19.60 4.53 4.80 20.51 4.33 15.06 20.92 4.25 25.84 21.724.09 6.44 23.30 3.82 12.46 28.28 3.16 2.88 33.16 2.70 2.22

The TGA curve of Form CS5 shows about 1.5% weight loss when heated to90° C., which is depicted in FIG. 3B.

The DSC curve of Form CS5 is depicted in FIG. 3C, which shows oneendothermic peak at around 91° C. (onset temperature).

Hygroscopicity Study of Form CS5

Dynamic vapor sorption (DVS) was applied to test hygroscopicity of FormCS5 with about 8 mg of samples. The results are listed in Table 3C. Theresult shows that weight gain of Form CS5 under 80% RH is 0.35%. FormCS5 is slightly hygroscopic according to the Chinese Pharmacopoeia 2015edition appendix XIX J Drug hygroscopic test guidelines. As depicted inFIG. 1I, weight gain of the solid disclosed in prior art under 80% RH is2.44%. The hygroscopicity of Form CS5 is lower than that of prior art.No form change of Form CS5 was observed before and after DVS test.

TABLE 3C Weight gain under 80% The definition of Sample RelativeHumidity hygroscopicity Form CS5 0.35% slightly hygroscopic Soliddisclosed in 2.44% hygroscopic CN100386339C

Example 4

Preparation of Form CS8 Example 1:

Form CS2 was stored at RT for one month. Then the sample was heated to95° C. at a rate of 10° C./min with nitrogen purging, held at 95° C. for2 min, and then cooled to RT at a rate of 5° C./min. The heating andcooling processes were conducted in a TGA equipment. The obtained samplewas stored at 60° C./75% RH for one month to get Form CS8. The XRPDpattern of Form CS8 is depicted in FIG. 4A, and the XRPD data are listedin Table 4A. The TGA curve of Form CS8 shows about 0.9% weight loss whenheated to 150° C., which is depicted in FIG. 4B. The DSC curve of FormCS8 is depicted in FIG. 4C, which shows one endothermic peak at around104° C.

TABLE 4A 2θ d spacing Intensity % 5.96 14.83 100.00 7.97 11.10 8.80 9.199.62 24.41 10.52 8.41 6.32 13.33 6.64 8.28 13.92 6.36 13.11 14.52 6.1020.11 15.50 5.72 82.15 15.76 5.62 46.17 16.67 5.32 21.95 17.18 5.1632.62 18.00 4.93 17.41 18.32 4.84 10.15 19.90 4.46 29.70 20.60 4.3178.93 21.71 4.09 66.13 22.80 3.90 28.42 22.96 3.87 27.23

Preparation of Form CS8 Example 2:

Form CS2 was stored at 25° C./60% RH for one week. The obtained samplewas heated to 95° C. at a rate of 10° C./min with nitrogen purging, heldat 95° C. for 2 min, and then cooled to RT at a rate of 10° C./min. Theheating and cooling processes were conducted in a TGA equipment. FormCS8 was obtained and the XRPD data are listed in Table 4B.

TABLE 4B 2θ d spacing Intensity % 4.09 21.62 7.54 5.99 14.75 100.00 8.0510.99 4.17 9.08 9.74 9.59 10.63 8.32 3.40 14.07 6.29 9.73 14.68 6.037.68 15.56 5.69 30.89 15.84 5.60 34.75 16.33 5.43 16.70 16.72 5.30 11.7217.20 5.16 15.53 17.73 5.00 17.76 19.95 4.45 15.46 20.67 4.30 22.0721.45 4.14 17.26 21.79 4.08 15.35 22.87 3.89 11.37

Stability Study of Form CS8

As active pharmaceutical ingredient plays an important part in drugproducts, it is vital that the crystalline active pharmaceuticalingredient has good physical and chemical stability. Good physicalstability avoids crystal transformation during the storage andformulation processes, thereby ensuring consistent and controllablequality of the drug substance and drug product.

Form CS8 were stored in open dishes under 25° C./60% RH, 40° C./75% RHand 60° C./75% RH conditions for three weeks, and sampled for XRPD test.The results showed that no form change was observed, and the XRPD dataof the sample before and after stored 60° C./75% RH for three weeks areshown in FIG. 4D as a representative.

Form CS8 is stable for at least three weeks under 25° C./60% RH, 40°C./75% RH and 60° C./75% RH. Further, Form CS8 is stable for at leastthree months. Further, Form CS8 is stable for at least six months.Further, Form CS8 is stable for at least one year. The results show thatForm CS8 has good stability and is suitable for drug development.

Hygroscopicity Study of Form CS8

Dynamic vapor sorption (DVS) was applied to test hygroscopicity of FormCS8 with about 7 mg of sample. The results were listed in Table 4C andDVS curve is shown in FIG. 5E. The result showed that the weight gain ofForm CS8 under 80% RH is 0.40%. Form CS8 is slightly hygroscopicaccording to the Chinese Pharmacopoeia 2015 edition appendix XIX J Drughygroscopic test guidelines. As depicted in FIG. 1I, weight gain of thesolid disclosed in prior art under 80% RH is 2.44%. The hygroscopicityof Form CS8 is lower than that of prior art. No form change of Form CS8was observed before and after DVS test. The XRPD patterns before andafter DVS were shown in FIG. 4F, top: the XRPD pattern before DVS;bottom: XRPD pattern after DVS.

TABLE 4C Weight gain under 80% The definition of Sample RelativeHumidity hygroscopicity Form CS8 0.40% Slightly hygroscopic Soliddisclosed in 2.44% Hygroscopic CN100386339C

The examples described above are only for illustrating the technicalconcepts and features of the present disclosure, and intended to makethose skilled in the art being able to understand the present disclosureand thereby implement it, and should not be concluded to limit theprotective scope of this disclosure. Any equivalent variations ormodifications according to the spirit of the present disclosure shouldbe covered by the protective scope of the present disclosure.

1. A crystalline form CS3 of compound (I), wherein the X-ray powderdiffraction pattern shows characteristic peaks at 2theta values of20.5°±0.2°, 15.4°±0.2° and 22.3°±0.2° using CuKα radiation.


2. The crystalline form CS3 according to claim 1, wherein the X-raypowder diffraction pattern shows one or two or three characteristicpeaks at 2theta values of 7.8°±0.2°, 17.0°±0.2° and 17.9°±0.2° usingCuKα radiation.
 3. The crystalline form CS3 according to claim 1,wherein the X-ray powder diffraction pattern shows one or two or threecharacteristic peaks at 2theta values of 8.4°±0.2°, 5.1°±0.2° and19.1°±0.2° using CuKα radiation.
 4. A process for preparing crystallineform CS3 according to claim 1, wherein the process comprises: 1)Suspending solid of compound (I) into a solvent mixture comprisingalcohols and water or alcohols and esters, then, stirring the solutionat 0-80° C. to obtain crystalline form CS3; or 2) Adding solid ofcompound (I) into a solvent to obtain a solution of compound (I), andadding an anti-solvent slowly into the solution of compound (I) insolvent, wherein the solvent is halogenated hydrocarbons, and theanti-solvent is aromatic hydrocarbons; or adding the solution ofcompound (I) in solvent into an anti-solvent, wherein the solvent isethers, and the anti-solvent is alkanes, stirring at room temperature toobtain crystalline form CS3.
 5. The process for preparing crystallineform CS3 according to claim 4, wherein in method 1) said mixture ofalcohols and water is mixture of isopropanol and water, said mixture ofalcohols and esters is mixture of isopropanol and isopropyl acetate, inmethod 2) said halogenated hydrocarbon is chloroform, said aromatichydrocarbon is toluene, said ether is tetrahydrofuran or2-methyltetrahydrofuran, said alkane is n-heptane.
 6. A crystalline formCS2 of compound (I), wherein the X-ray powder diffraction pattern showscharacteristic peaks at 2theta values of 20.5°±0.2°, 16.8°±0.2° and4.3°±0.2° using CuKα radiation.
 7. The crystalline form CS2 according toclaim 6, wherein the X-ray powder diffraction pattern shows one or twoor three characteristic peaks at 2theta values of 8.4°±0.2°, 17.6°±0.2°and 14.9°±0.2° using CuKα radiation.
 8. A process for preparingcrystalline form CS2 according to claim 6, wherein the processcomprises: 1) Mixing solid of compound (I) with methanol or a solventmixture of ethanol and acetone, filtering after dissolved, storing thefiltrate in a larger sealed device containing acetonitrile at 0-30° C.to obtain crystalline form CS2; or 2) Suspending solid of compound (I)into acetonitrile, then stirring the solution at 0-30° C. to obtaincrystalline form CS2.
 9. A crystalline form CS5 of compound (I), whereinthe X-ray powder diffraction pattern shows characteristic peaks at2theta values of 4.8°±0.2°, 9.7°±0.2° and 6.4°±0.2° using CuKαradiation.
 10. The crystalline form CS5 according to claim 9, whereinthe X-ray powder diffraction pattern shows one or two or three or fourcharacteristic peaks at 2theta values of 20.9°±0.2°, 16.8°±0.2°,23.3°±0.2° and 14.6°±0.2° using CuKα radiation.
 11. A process forpreparing crystalline form CS5 according to claim 9, wherein the processcomprises: Adding solid of compound (I) into a solvent selected fromalcohols to obtain a solution of compound (I), and adding acetonitrileslowly into the solution of compound (I), stirring at room temperatureuntil solid precipitates, then, separating and drying to obtaincrystalline form CS5.
 12. The process for preparing crystalline form CS5according to claim 11, wherein said alcohols are methanol, ethanol andisopropanol.
 13. A crystalline form CS8 of compound (I), wherein theX-ray powder diffraction pattern shows characteristic peaks at 2thetavalues of 6.0°±0.2°, 15.5°±0.2° and 20.7°±0.2° using CuKα radiation. 14.The crystalline form CS8 according to claim 13, wherein the X-ray powderdiffraction pattern shows one or two or three or four characteristicpeaks at 2theta values of 21.8°±0.2°, 9.1°±0.2°, 19.9°±0.2° and22.9°±0.2° using CuKα radiation.
 15. A process for preparing crystallineform CS8 according to claim 13, wherein the process comprises: Storingcrystalline form CS2 at room temperature for 7-30 days, heating theobtained sample to 95° C.±2° C. at a rate of 5-20° C./min with nitrogenpurging, holding at 95° C.±2° C. for 1-10 min, and then cooling to roomtemperature at a rate of 5-20° C./min to obtain crystalline form CS8.16. A pharmaceutical composition, wherein said pharmaceuticalcomposition comprises a therapeutically effective amount of crystallineform CS3 according to claim 1, and pharmaceutically acceptable carriers,diluents or excipients.
 17. A method for treating non-alcoholicsteatohepatitis, gallstones, cholesterol gallstone, or atherosclerosis,comprising administering to a patient in need thereof a therapeuticallyeffective amount of crystalline form CS3 according to claim
 1. 18.(canceled)
 19. A method for treating non-alcoholic steatohepatitis,gallstones, cholesterol gallstone, or atherosclerosis, comprisingadministering to a patient in need thereof a therapeutically effectiveamount of crystalline form CS2 according to claim
 6. 20. A method fortreating non-alcoholic steatohepatitis, gallstones, cholesterolgallstone, or atherosclerosis, comprising administering to a patient inneed thereof a therapeutically effective amount of crystalline form CS5according to claim
 9. 21. A method for treating non-alcoholicsteatohepatitis, gallstones, cholesterol gallstone, or atherosclerosis,comprising administering to a patient in need thereof a therapeuticallyeffective amount of crystalline form CS8 according to claim 13.